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| Registros recuperados: 13 | |
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| Geslin, Claire; Gaillard, Melusine; Flament, Didier; Rouault, Karen; Le Romancer, Marc; Prieur, Daniel; Erauso, Gael. |
| Only one virus-like particle (VLP) has been reported from hyperthermophilic Euryarchaeotes. This VLP, named PAV1 is shaped like a lemon and was isolated from a strain of "Pyrococcus abyssi," a deep-sea isolate. Its genome consists of a double-stranded circular DNA of 18 kb which is also present at a high copy number (60 per chromosome) free within the host cytoplasm but is not integrated into the host chromosome. Here, we report the results of complete analysis of the PAV1 genome. All the 25 predicted genes, except 3, are located on one DNA strand. A transcription map has been made by using a reverse transcription-PCR assay. All the identified open reading frames (ORFs) are transcribed. The most significant similarities relate to four ORFs. ORF 180a shows... |
| Tipo: Text |
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| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-3536.pdf |
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| Roussel, Erwan; Sauvadet, Anne-laure; Chaduteau, Carine; Fouquet, Yves; Charlou, Jean-luc; Prieur, Daniel; Cambon, Marie-anne. |
| P>The distribution of the archaeal communities in deep subseafloor sediments [0-36 m below the seafloor (mbsf)] from the New Caledonia and Fairway Basins was investigated using DNA- and RNA-derived 16S rRNA clone libraries, functional genes and denaturing gradient gel electrophoresis (DGGE). A new method, Co-Migration DGGE (CM-DGGE), was developed to access selectively the active archaeal diversity. Prokaryotic cell abundances at the open-ocean sites were on average similar to 3.5 times lower than at a site under terrestrial influence. The sediment surface archaeal community (0-1.5 mbsf) was characterized by active Marine Group 1 (MG-1) Archaea that co-occurred with ammonia monooxygenase gene (amoA) sequences affiliated to a group of uncultured... |
| Tipo: Text |
Palavras-chave: Marine subsurface sediments; Sea floor biosphere; Gradient gel electrophoresis; Microbial communities; Molecular diversity; Anaerobic oxidation; Sequence alignment; Phylogenetic trees; Extracellular DNA; Methane hydrate. |
| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/2009/publication-6801.pdf |
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| Roussel, Erwan; Konn, Cecile; Charlou, Jean-luc; Donval, Jean-pierre; Fouquet, Yves; Querellou, Joel; Prieur, Daniel; Cambon-bonavita, Marie-anne. |
| The distribution of Archaea and methanogenic, methanotrophic and sulfate-reducing communities in three Atlantic ultramafic-hosted hydrothermal systems (Rainbow, Ashadze, Lost City) was compared using 16S rRNA gene and functional gene (mcrA, pmoA and dsrA) clone libraries. The overall archaeal community was diverse and heterogeneously distributed between the hydrothermal sites and the types of samples analyzed (seawater, hydrothermal fluid, chimney and sediment). The Lost City hydrothermal field, characterized by high alkaline warm fluids (pH>11; T<95 °C), harbored a singular archaeal diversity mostly composed of unaffiliated Methanosarcinales. The archaeal communities associated with the recently discovered Ashadze 1 site, one of the deepest active... |
| Tipo: Text |
Palavras-chave: Archaea; Hydrothermal vent; Mid-Atlantic Ridge; 16S rRNA gene; Sediment; Ultramafic. |
| Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00067/17836/15573.pdf |
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| Prieur, Daniel. |
| Le contrôle bactérien des élevages de mollusques bivalves a été entrepris dans notre laboratoire depuis 1972 avec l'aide financière du C.N.E.X.O. (Contrats 72/489, 73/725, 74/794, 75/1198, 76/1434, 77/1626, 78/1835, 79/2048, 80/2308). Un bref rappel des résultats obtenus de 1972 à 1979 a été présenté en préambule au programme scientifique du contrat 80/2308. Ce rappel avait pour but de tracer l'évolution des axes de recherche et la progression des connaissances depuis le début de ce travail. Ces résultats obtenus ont permis la rédaction de vingt deux articles et communications à divers congrès, la soutenance d'une thèse de 3ème cycle et d'une thèse de Doctorat d'Etat. La liste complète de ces publications est jointe en annexe à ce document. Nous ne... |
| Tipo: Text |
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| Ano: 1981 |
URL: http://archimer.ifremer.fr/doc/00151/26200/24220.pdf |
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| Byrne, Nathalie; Lesongeur, Francoise; Bienvenu, Nadege; Geslin, Claire; Alain, Karine; Prieur, Daniel; Godfroy, Anne. |
| Both cultivation and molecular techniques were used to investigate the microbial diversity and dynamic of a deep-sea vent chimney. The enrichment cultures performed in a gas-lift bioreactor were inoculated with a black smoker chimney sample collected on TAG site on the mid-Atlantic ridge. To mimic as close as possible environmental conditions, the cultures were performed in oligotrophic medium with nitrogen, hydrogen and carbon dioxide (N-2/H-2/CO2) gas sweeping. Also, the temperature was first settled at a temperature of 85A degrees C and colloidal sulphur was added. Then, the temperature was lowered to 60A degrees C and sulphur was omitted. Archaeal and bacterial diversity was studied in both culture and natural samples. Through 16S rRNA gene sequences... |
| Tipo: Text |
Palavras-chave: 16S rRNA genes; Oligotrophic condition; Environmental condition; Bioreactor; Continuous enrichment culture; Microbial diversity; Deep sea hydrothermal vent. |
| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/2009/publication-6920.pdf |
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| Prieur, Daniel. |
| The bacterial colonization of the digestive tract of Mytilus edulis was studied, in the laboratory, using juvenile mussels. For these experiments, six bacterial strains belonging to four different taxonomic groups, were selected from the author's bacterial collection. The results were obtained by examination of histological sections of mussels using both light and scanning electron microscopy. All the selected strains were ingested by the mussels, and observed at different levels of the digestive tract as early as 15 minutes after the distribution of the bacterial suspension. Some strains were able to form microcolonies (5 to 10 um in diamater) and this probably made easier the ingestion. The bacterial cells were also agglomerated by the bivalve's mucus,... |
| Tipo: Text |
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| Ano: 1982 |
URL: http://archimer.ifremer.fr/doc/00306/41748/40959.pdf |
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| Prieur, Daniel. |
| During the Biocyarise 1984 cruise, individuals of Alvinella pompejana and Bathymodiolus thermophilus were sampled by the submersible Cyana, on the 13 degree N site. Seventy-three heterotrophic bacterial strains were isolated from different body regions of these 2 animals. All the strains are Gram negative rods, most of them are motile. The bacteria isolated from Alvinella epidermis showed the highest index of carbohydrate and amino-acid utilization as sole carbon source. The thermal tolerance of these bacteria is well correlated to the temperatures of the biotopes of these invertebrates. The possible role of these heterotrophic bacteria in the hydrothermal ecosystem is discussed. |
| Tipo: Text |
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| Ano: 1988 |
URL: http://archimer.ifremer.fr/doc/00267/37828/35909.pdf |
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| Toffin, Laurent; Zink, Klaus; Kato, Chiaki; Pignet, Patricia; Bidault, Adeline; Bienvenu, Nadège; Birrien, Jean-louis; Prieur, Daniel. |
| A piezotolerant, mesophilic, marine lactic acid bacterium (strain LT20(T)) was isolated from a deep sub-seafloor sediment core collected at Nankai Trough, off the coast of Japan. Cells were Gram-positive, rod-shaped, non-sporulating and non-motile. The NaGl concentration range for growth was 0-120 gl(-1), with the optimum at 10-20 g l(-1). The temperature range for growth at PH 7.0 was 4-50 degreesC, with the optimum at 37-40 degreesC. The optimum pH for growth was 7.0-8.0. The optimum pressure for growth was 0.1 MPa with tolerance up to 30 MPa. The main cellular phospholipids were phosphatidylglycerols (25 %), diphosphatidylglycerols (34 %) and a group of compounds tentatively identified as ammonium-containing phosphatidylserines (32 %);... |
| Tipo: Text |
Palavras-chave: Phylogeny; Biochemistry; Microbiology; Thermophilic bacteria; Deep sub seafloor sediment. |
| Ano: 2005 |
URL: http://archimer.ifremer.fr/doc/2005/publication-592.pdf |
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| Alain, Karine; Callac, Nolwenn; Guegan, Marianne; Lesongeur, Francoise; Crassous, Philippe; Cambon-bonavita, Marie-anne; Querellou, Joel; Prieur, Daniel. |
| A novel strictly anaerobic, thermophilic, sulfur-reducing bacterium, designated PH1209(T), was isolated from an East Pacific Rise hydrothermal vent (13 degrees N) sample and studied using a polyphasic taxonomic approach. Cells were Gram-negative, motile rods (approx. 1.60x0.40 mu m) with a single polar flagellum. Strain PH1209(T) grew at temperatures between 33 and 65 degrees C (optimum 60 degrees C), from pH 5.0 to 8.0 (optimum 6.0-6.5), and between 2 and 4% (w/v) NaCl (optimum 3%). Cells grew chemolithoautotrophically with H-2 as an energy source, So as an electron acceptor and CO2 as a carbon source. Strain PH1209(T) was also able to use peptone and yeast extract as carbon sources. The G+C content of the genomic DNA was 35 mol%. Phylogenetic: analyses... |
| Tipo: Text |
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| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/2009/publication-6585.pdf |
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| Byrne, Nathalie; Strous, Marc; Crepeau, Valentin; Kartal, Boran; Birrien, Jean-louis; Schmid, Markus; Lesongeur, Francoise; Schouten, Stefan; Jaeschke, Andrea; Jetten, Mike; Prieur, Daniel; Godfroy, Anne. |
| Recent studies indicate that ammonia is an important electron donor for the oxidation of fixed nitrogen, both in the marine water column and sediments. This process, known as anammox, has so far only been observed in a large range of temperature habitats. The present study investigated the role of anammox in hydrothermal settings. During three oceanographic expeditions to the Mid-Atlantic Ridge, hydrothermal samples were collected from five vent sites, at depths ranging from 750 to 3650m from cold to hot habitats. Evidence for the occurrence of anammox in these particular habitats was demonstrated by concurrent surveys, including the amplification of 16S rRNA gene sequences related to known anammox bacteria, ladderanes lipids analysis and measurement of a... |
| Tipo: Text |
Palavras-chave: Hydrothermal vent; Ladderanes; 16S rRNA; Activity; Micro organisms; Anammox. |
| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/2009/publication-6921.pdf |
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| Zeng, Xiang; Birrien, Jean-louis; Fouquet, Yves; Cherkashov, Georgy; Jebbar, Mohamed; Querellou, Joel; Oger, Philippe; Cambon-bonavita, Marie-anne; Xiao, Xiang; Prieur, Daniel. |
| A novel hydrothermal site was discovered in March 2007, on the mid-Atlantic ridge during the cruise 'Serpentine'. At a depth of 4100 m, the site 'Ashadze' is the deepest vent field known so far. Smoker samples were collected with the ROV 'Victor 6000' and processed in the laboratory for the enrichment of anaerobic heterotrophic microorganisms under high-temperature and high-hydrostatic pressure conditions. Strain CH1 was successfully isolated and assigned to the genus Pyrococcus, within the Euryarchaeota lineage within the Archaea domain. This organism grows within a temperature range of 80 to 108 degrees C and a pressure range of 20 to 120 MPa, with optima for 98 degrees C and 52 MPa respectively. Pyrococcus CH1 represents the first obligate piezophilic... |
| Tipo: Text |
Palavras-chave: Thermococcales; Extremophile; Deep sea hydrothermal vent; High hydrostatic pressure; Archaea. |
| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/2009/publication-6812.pdf |
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| Toffin, Laurent; Bidault, Adeline; Pignet, Patricia; Tindall, Brian; Slobodkin, Alexander; Kato, Chiaki; Prieur, Daniel. |
| A novel piezotolerant, mesophilic, facultatively anaerobic, organotrophic, polarly flagellated bacterium (strain LT13a(T)) was isolated from a deep sediment layer in the Nankai Trough (Leg 190, Ocean Drilling Program) off the coast of Japan. This organism used a wide range of organic substrates as sole carbon and energy sources: pyruvate, glutamate, succinate, fumarate, lactate, citrate, peptone and tryptone. Oxygen, nitrate, fumarate, ferric iron and cystine were used as electron acceptors. Maximal growth rates were observed at a hydrostatic pressure of 10 MPa. Hydrostatic pressure for growth was in the range 0.1-50 MPa. Predominant cellular fatty acids were 16:1 omega7c, 15: 0 iso, 16: 0 and 13: 0 iso. The G + C content of the DNA was 44.9 mol%. On the... |
| Tipo: Text |
Palavras-chave: Phylogeny; Bacteria; Microbiology; Deep sediment layer. |
| Ano: 2004 |
URL: http://archimer.ifremer.fr/doc/2004/publication-590.pdf |
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| Jeanthon, Christian; L' Haridon, Ste! Phane; Cueff, Valerie; Banta, Amy; Reysenbach, Anna-louise; Prieur, Daniel. |
| A thermophillic, non-spore-forming, marine, sulfate-reducing bacterium, strain SL6(T), was isolated from deep-sea hydrothermal sulfides collected at Guaymas Basin. The Gram-negative-staining cells occurred singly or in pairs as small, highly motile rods. The temperature range for growth was 50-80degreesC with an optimum at 75degreesC. The pH range for growth at 70degreesC was 6.3-6.8, with an optimum at 6.5. The NaCl concentration range for growth was 5.55 g l(-1), with an optimum at 30 g l(-1). H-2 and CO2 were the only substrates for growth and sulfate reduction. However, growth was stimulated by several organic compounds. Sulfur, thiosulfate, sulfite, cystine, nitrate and fumarate were not used as electron acceptors. Pyruvate, lactate and malate did not... |
| Tipo: Text |
Palavras-chave: Sulfate reduction; Thermodesulfobacterium; Bacteria; Thermophile; Deep sea hydrothermal vent. |
| Ano: 2002 |
URL: https://archimer.ifremer.fr/doc/2002/publication-585.pdf |
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| Registros recuperados: 13 | |
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